Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia
Identifieur interne : 005B50 ( Main/Exploration ); précédent : 005B49; suivant : 005B51Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia
Auteurs : Patrick C. Y. Woo [Hong Kong] ; Susanna K. P. Lau [Hong Kong] ; Beatrice H. L. Wong [Hong Kong] ; Hoi-Wah Tsoi [Hong Kong] ; Ami M. Y. Fung [Hong Kong] ; Kwok-Hung Chan [Hong Kong] ; Victoria K. P. Tam [Hong Kong] ; J. S. Malik Peiris [Hong Kong] ; Kwok-Yung Yuen [Hong Kong]Source :
- Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2004.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (sang), Antigènes viraux, Humains, Immunoglobuline A (sang), Immunoglobuline G (sang), Immunoglobuline M (sang), Protéines nucléocapside (immunologie), Sensibilité et spécificité, Spécificité des anticorps, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (immunologie), Test ELISA (), Tests sérologiques (), Virus du SRAS (immunologie), Études cas-témoins.
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- immunologie : Protéines nucléocapside, Syndrome respiratoire aigu sévère, Virus du SRAS.
- sang : Anticorps antiviraux, Immunoglobuline A, Immunoglobuline G, Immunoglobuline M.
- Pascal (Inist)
English descriptors
- KwdEn :
- Antibodies, Viral (blood), Antibody, Antibody Specificity, Antigens, Viral, Case-Control Studies, Coronavirus, Detection, Enzyme-Linked Immunosorbent Assay (methods), Enzyme-Linked Immunosorbent Assay (statistics & numerical data), Humans, Immunoglobulin A (blood), Immunoglobulin G (blood), Immunoglobulin M (blood), Microbiology, Nucleocapsid, Nucleocapsid Proteins (immunology), Pneumonia, Protein, SARS Virus (immunology), Sensitivity and Specificity, Serologic Tests (methods), Serologic Tests (statistics & numerical data), Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (immunology), Severe acute respiratory syndrome.
- MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin A, Immunoglobulin G, Immunoglobulin M.
- chemical , immunology : Nucleocapsid Proteins.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- methods : Enzyme-Linked Immunosorbent Assay, Serologic Tests.
- statistics & numerical data : Enzyme-Linked Immunosorbent Assay, Serologic Tests.
- Antibody Specificity, Antigens, Viral, Case-Control Studies, Humans, Sensitivity and Specificity.
Abstract
We report the evaluation of recombinant severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) nucleocapsid protein enzyme-linked immunosorbent assay (ELISA)-based antibody tests for serodiagnosis of SARS-CoV pneumonia and compare the sensitivities and specificities of this ELISA for detection of immunoglobulin G (IgG), IgM, IgA, and their combinations with serum samples from 149 healthy blood donors who donated blood 3 years ago as controls and 106 SARS-CoV pneumonia patients in Hong Kong. The specificities of the ELISA for IgG, IgM, and IgA detection were 95.3, 96.6, and 96.6%, respectively, with corresponding sensitivities of 943, 59.4, and 60.4%, respectively. The present ELISA appears to be a sensitive test for serodiagnosis of SARS-CoV pneumonia, is much more economical and less labor-intensive than the indirect immunofluorescence assay, and does not require cultivation of SARS-CoV.
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Y." last="Woo">Patrick C. Y. Woo</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, University of Hong Kong, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, University of Hong Kong, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K. P." last="Lau">Susanna K. P. Lau</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, University of Hong Kong, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, University of Hong Kong, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Wong, Beatrice H L" sort="Wong, Beatrice H L" uniqKey="Wong B" first="Beatrice H. L." last="Wong">Beatrice H. L. Wong</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, University of Hong Kong, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, University of Hong Kong, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Tsoi, Hoi Wah" sort="Tsoi, Hoi Wah" uniqKey="Tsoi H" first="Hoi-Wah" last="Tsoi">Hoi-Wah Tsoi</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, University of Hong Kong, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, University of Hong Kong, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Fung, Ami M Y" sort="Fung, Ami M Y" uniqKey="Fung A" first="Ami M. Y." last="Fung">Ami M. Y. Fung</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, University of Hong Kong, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, University of Hong Kong, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, University of Hong Kong, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, University of Hong Kong, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Tam, Victoria K P" sort="Tam, Victoria K P" uniqKey="Tam V" first="Victoria K. P." last="Tam">Victoria K. P. Tam</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, University of Hong Kong, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, University of Hong Kong, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J. S. Malik" last="Peiris">J. S. Malik Peiris</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, University of Hong Kong, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, University of Hong Kong, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, University of Hong Kong, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, University of Hong Kong, Queen Mary Hospital</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of clinical microbiology : (Print)</title><title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title><idno type="ISSN">0095-1137</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of clinical microbiology : (Print)</title><title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title><idno type="ISSN">0095-1137</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (blood)</term><term>Antibody</term><term>Antibody Specificity</term><term>Antigens, Viral</term><term>Case-Control Studies</term><term>Coronavirus</term><term>Detection</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Enzyme-Linked Immunosorbent Assay (statistics & numerical data)</term><term>Humans</term><term>Immunoglobulin A (blood)</term><term>Immunoglobulin G (blood)</term><term>Immunoglobulin M (blood)</term><term>Microbiology</term><term>Nucleocapsid</term><term>Nucleocapsid Proteins (immunology)</term><term>Pneumonia</term><term>Protein</term><term>SARS Virus (immunology)</term><term>Sensitivity and Specificity</term><term>Serologic Tests (methods)</term><term>Serologic Tests (statistics & numerical data)</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe acute respiratory syndrome</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux (sang)</term><term>Antigènes viraux</term><term>Humains</term><term>Immunoglobuline A (sang)</term><term>Immunoglobuline G (sang)</term><term>Immunoglobuline M (sang)</term><term>Protéines nucléocapside (immunologie)</term><term>Sensibilité et spécificité</term><term>Spécificité des anticorps</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Test ELISA ()</term><term>Tests sérologiques ()</term><term>Virus du SRAS (immunologie)</term><term>Études cas-témoins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin A</term><term>Immunoglobulin G</term><term>Immunoglobulin M</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Protéines nucléocapside</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term><term>Serologic Tests</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Immunoglobuline A</term><term>Immunoglobuline G</term><term>Immunoglobuline M</term></keywords><keywords scheme="MESH" qualifier="statistics & numerical data" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term><term>Serologic Tests</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Antibody Specificity</term><term>Antigens, Viral</term><term>Case-Control Studies</term><term>Humans</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Antigènes viraux</term><term>Coronavirus</term><term>Détection</term><term>Anticorps</term><term>Humains</term><term>Nucléocapside</term><term>Protéine</term><term>Microbiologie</term><term>Pneumonie</term><term>Sensibilité et spécificité</term><term>Spécificité des anticorps</term><term>Syndrome respiratoire aigu sévère</term><term>Test ELISA</term><term>Tests sérologiques</term><term>Études cas-témoins</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We report the evaluation of recombinant severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) nucleocapsid protein enzyme-linked immunosorbent assay (ELISA)-based antibody tests for serodiagnosis of SARS-CoV pneumonia and compare the sensitivities and specificities of this ELISA for detection of immunoglobulin G (IgG), IgM, IgA, and their combinations with serum samples from 149 healthy blood donors who donated blood 3 years ago as controls and 106 SARS-CoV pneumonia patients in Hong Kong. The specificities of the ELISA for IgG, IgM, and IgA detection were 95.3, 96.6, and 96.6%, respectively, with corresponding sensitivities of 943, 59.4, and 60.4%, respectively. The present ELISA appears to be a sensitive test for serodiagnosis of SARS-CoV pneumonia, is much more economical and less labor-intensive than the indirect immunofluorescence assay, and does not require cultivation of SARS-CoV.</div></front></TEI><affiliations><list><country><li>Hong Kong</li></country></list><tree><country name="Hong Kong"><noRegion><name sortKey="Woo, Patrick C Y" sort="Woo, Patrick C Y" uniqKey="Woo P" first="Patrick C. Y." last="Woo">Patrick C. Y. Woo</name></noRegion><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><name sortKey="Fung, Ami M Y" sort="Fung, Ami M Y" uniqKey="Fung A" first="Ami M. Y." last="Fung">Ami M. Y. Fung</name><name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K. P." last="Lau">Susanna K. P. Lau</name><name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J. S. Malik" last="Peiris">J. S. Malik Peiris</name><name sortKey="Tam, Victoria K P" sort="Tam, Victoria K P" uniqKey="Tam V" first="Victoria K. P." last="Tam">Victoria K. P. Tam</name><name sortKey="Tsoi, Hoi Wah" sort="Tsoi, Hoi Wah" uniqKey="Tsoi H" first="Hoi-Wah" last="Tsoi">Hoi-Wah Tsoi</name><name sortKey="Wong, Beatrice H L" sort="Wong, Beatrice H L" uniqKey="Wong B" first="Beatrice H. L." last="Wong">Beatrice H. L. Wong</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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